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research interest

Using cellular resolution recording techniques, we address fundamental questions concerned with the formation, initiation, and progression of epileptic seizures in the intact brain. Epileptic seizures likely arise through the aberrant interaction of local neural populations whose underlying circuitry is not well understood. Despite >100 years of research, even the most basic aspects of epilepsy have escaped our understanding, and up to 30% of patients remain unresponsive to pharmacotherapy. No clear answer exists as to how an epileptic network forms over time, how individual seizures start or terminate, why seizures remain focal in one case, or spread in another. One reason for this persistent lack of understanding has been the technical difficulty to study densely packed epileptic networks at sufficient temporal and spatial resolution. During his post-doc at Columbia University, Dr. Wenzel studied acute focal seizure formation and spread at cellular resolution within and across cortical layers in vivo. His newly formed group at Bonn University combines cutting edge cellular scale chronic in vivo calcium imaging with electrophysiology, and behavioral assessment in various models of chronic epilepsy in mice. The core goal of the group is the identification of basic local circuit elements of chronically seizure producing networks that in the best case are shared across different types of epilepsy.

Experimental techniques

Two-photon in vivo imaging of seizure microprogression
Local cortical network recruitment patterns during seizure microprogression
Cellular scale in vivo imaging of neural subtypes
green: Pyramidal cells (PYR) magenta: PV interneurons
recording_ca_lfp_sharp_fig_cut2
Multimodal recordings across anatomical scales
in vivo
Two-photon Ca2+ imaging +
Local field potential recordings + Intracellular nanosharp recordings
In vivo imaging across cortical layers and in hippocampus
Glass microprism-, or micro-cylinder-assisted multilayer or hippocampal imaging

LAB MEMBERS

Michael Wenzel, M.D.

Since July 2019 – Bonfor young investigator award
Since July 2018 – Department of Epileptology, UKB Bonn, Clinical training in Neurology
2014 – 2018 Post-doc with Rafael Yuste at Columbia University, New York
2011 – 2013 Department of Neurology, LMU, Clinical training in Neurology
2007 – 2009 Doctoral research program Molecular Medicine, LMU, Munich
2003 – 2010 Medical School, LMU, Munich

We just started:) This section will be updated soon!

Collaborators

Dr. Heinz Beck (In vivo two-photon imaging, experimental epileptology)

Institute for Experimental Epileptology and Cognition Research, Bonn University, Germany

Dr. Dr. Florian Mormann (Single unit recordings in humans) 

Department of Epileptology, Bonn University, Germany

Dr. Laura Ewell (Hippocampal memory coding, experimental epileptology)

Institute for Experimental Epileptology and Cognition Research, Bonn University, Germany

Dr. Tony Kelly (Hippocampal dendritic integration, experimental epileptology)

Institute for Experimental Epileptology and Cognition Research, Bonn University, Germany

Dr. Darcy Peterka (In vivo two-photon imaging)

Zuckerman Mind Brain Behavior Institute, Columbia University, New York, USA

Dr. Krishna Jayant (Nanoelectronics, biomedical engineering) 

Weldon School of Biomedical Engineering, Purdue University, Indiana, USA

Dr. Yuki Bando (Molecular Biology, Voltage Imaging)

Hamamatsu University School of Medicine, Hamamatsu, Japan

publications

Wenzel M, Hamm JP, Peterka DS, Yuste R. Acute focal seizures start as local synchronizations of neuronal ensembles. Journal of Neuroscience (2019) doi.org/10.1523/JNEUROSCI.3176-18.2019

Wenzel M*, Han S*, Smith EH*, Hoel E, Greger B, House PA, Yuste R. Reduced repertoire of cortical microstates and neuronal ensembles in medically induced loss of consciousness. Cell Systems (2019) 8, 467–474; *equal contribution

Jayant K*, Wenzel M* , Hamm JP, Bando Y, Sahin O, Shepard KL, Yuste R. Flexible nanopipettes for minimally invasive intracellular electrophysiology in vivo. Cell Reports  (2019) 26, 266–278; *equal contribution

Liou JY*, Ma H*, Wenzel M, Zhao M, Baird-Daniel E, Smith EH, Daniel AGS, Emerson R, Yuste R, Schwartz TH, Schevon CA. Role of inhibitory control in modulating focal seizure spread. Brain (2018) 141, Issue 7, 2083–2097; *equal contr.

Wenzel M, Hamm JP, Peterka DS, Yuste R. Reliable and elastic propagation of cortical seizures in vivo. Cell Reports (2017) 19:2681–2693

Baird-Daniel E*, Daniel AGS*, Wenzel M, Li D, Liou JY, Laffont P, Zhao M, Yuste R, Ma H, Schwartz TH. Glial waves are triggered by seizure activity and not essential for initiating ictal onset or neurovascular coupling. Cerebral Cortex (2017) 27:3318-3330; *equal contribution

Ricci S, Grandgirard D, Wenzel M, Braccini T, Salvatore P, Oggioni MR, Leib SL, Koedel U. Inhibition of matrix metalloproteinases attenuates brain damage in experimental meningococcal meningitis. BMC Infect Dis. (2014) 14:726

Wenzel M, Jakob L, Wieser A, Schauber J, Dimitriadis K, Schubert S, Pfister HW. Corticosteroid-Induced Meningococcal Meningitis in a Patient With Chronic Meningococcemia. JAMA Dermatology (2014)150(7):752-755

Heinrich D, Wenzel M, Dimitriadis K, Mühlstädt M. An international medical curriculum – first steps of implementation. Medical Education (2013) 47(5):516

Höhne C, Wenzel M, Angele B, Hammerschmidt S, Häcker H, Klein M, Bierhaus A, Sperandio M, Pfister HW, Koedel U. HMGB1 prolongs inflammation and worsens disease in experimental pneumococcal meningitis. Brain (2013) 136:1746-59

Wenzel M, Wunderlich M, Besch R, Poeck H, Willms S, Schwantes A, Kremer M, Sutter G, Endres S, Schmidt A, Rothenfusser S. Cytosolic DNA triggers mitochondrial apoptosis via DNA damage signaling proteins independently of AIM2 and RNA polymerase III. J Immunology (2012) 188(1):394-403

Schmidt A, Schwerd T, Hamm W, Hellmuth JC, Cui S, Wenzel M, Hoffmann FS, Michallet MC, Besch R, Hopfner KP, Endres S, Rothenfusser S. 5′-triphosphate RNA requires base-paired structures to activate antiviral signaling via RIG-I. PNAS (2009) 106(29):12067-72 

Contact

Dr. Michael Wenzel
michael.wenzel@ukbonn.de

 

Department of Epileptology

University Hospital Bonn
Venusberg-Campus 1
Building 83
D-53127 Bonn
Germany